Mouse Carbonic anhydrase 3 (Ca3) ELISA Kit

A Sandwich ELISA Kit from Assay Genie designed for the colorimetric detection of carbonic anhydrase 3 levels in mouse samples. Intended for research use only, the assay is formulated to deliver high sensitivity and specificity for dependable and consistent results. Carbonic anhydrase 3 is an enzyme involved in the regulation of acid-base balance and electrolyte transport in tissues including the liver and muscle, with its dysregulation linked to metabolic, liver, and muscle disorders.

Validated for serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, the kit detects natural and recombinant mouse carbonic anhydrase 3 (UniProt P16015), a 29,366 Da cytoplasmic protein that catalyses the reversible hydration of carbon dioxide. Manufacturer-stated performance includes a detection range of 0.312-20ng/mL and a sensitivity of 0.178ng/mL, with an intra-assay CV of 5.3% and an inter-assay CV of 7.7%. Linear dilution recovery in serum across 1:2 to 1:16 spans 83% to 119%, with average serum recovery of 94% and average plasma recovery of 96%. The target is also referred to as Carbonate dehydratase III, Carbonic anhydrase III, CA-III, and Car3.