Mouse cAMP-dependent protein kinase catalytic subunit alpha (Prkaca) ELISA Kit

A sandwich ELISA kit from Assay Genie for the quantitative detection of mouse cAMP-dependent protein kinase catalytic subunit alpha (Prkaca), also known as PKA C-alpha. The assay is reactive with mouse samples and is suitable for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is stated for natural and recombinant mouse Prkaca.

Reported performance characteristics include a detection range of 0.312-20 ng/mL, a sensitivity of 0.159 ng/mL, an intra-assay CV of 7.3% and an inter-assay CV of 8.9%. Spike-recovery averages are reported as 85% (range 80-91%) for serum and 87% (range 81-93%) for plasma, and linearity has been assessed in serum, EDTA plasma and heparin plasma across dilution factors of 1:2 to 1:16. The associated UniProt record is P05132, and the product is for research use only.

Prkaca is described as a key regulatory enzyme of the cAMP signaling pathway, influencing metabolism, gene expression and cell growth, with dysregulation implicated in cancer, diabetes and cardiovascular disorders, placing it within cardiovascular research applications.