Mouse Calcitonin ELISA Kit

Designed for the precise quantification of calcitonin levels in mouse serum, plasma, and tissue homogenates, this Sandwich ELISA, Double Antibody kit from Assay Genie offers reliable performance for bone health and endocrine disorder research. Calcitonin is described as a key hormone that regulates calcium levels in the body and plays a vital role in bone metabolism, making it a critical biomarker for studying conditions such as osteoporosis and Paget's disease.

Acceptable sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit reports a sensitivity of 9.375 pg/mL and a detection range of 15.625-1000 pg/mL. Storage is recommended at 2-8°C for 12 months.

Linearity has been demonstrated for serum (85-102% at 1:2, 88-98% at 1:4, 84-99% at 1:8), EDTA plasma (89-101% at 1:2, 84-96% at 1:4, 93-99% at 1:8), and heparin plasma (87-102% at 1:2, 83-100% at 1:4, 80-99% at 1:8). Recovery ranges are 89-105% for serum (average 97%), 88-105% for EDTA plasma (average 95%), and 86-104% for heparin plasma (average 94%). The procedure involves a primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, and HRP-streptavidin binding for 30 minutes, followed by color development with TMB substrate and absorbance reading at 450 nm.