Mouse Calbindin/CALB1 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit uses a double-antibody, colorimetric method for the precise quantification of calbindin (CALB1) levels in mouse samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Calbindin is a calcium-binding protein encoded by the CALB1 gene that contributes to calcium homeostasis and neuronal function, and its dysregulation has been linked to neurological disorders such as Alzheimer's disease, Parkinson's disease, and epilepsy.

The assay has a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL, and the kit is intended to be stored at 2-8°C for 12 months. Linearity data for serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8 are reported alongside recovery data across the same sample types. The protocol involves primary incubation at 37°C, detection antibody binding, HRP-streptavidin binding, TMB color development, and absorbance reading at 450 nm, with sample-specific preparation steps such as clotting and centrifugation for serum, anticoagulant collection and centrifugation for plasma, homogenization for tissue, and lysis for cells.

Abnormalities in calbindin expression have been associated with Alzheimer's disease, Parkinson's disease, and epilepsy, making the kit a tool for researchers studying the role of CALB1 in health and disease. Sample preparation notes are provided for serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other liquid samples, with additional guidance available from Assay Genie's technical support team.