Mouse CA9 (Carbonic anhydrase 9) ELISA Kit

Designed by Assay Genie for the quantitative detection of Carbonic Anhydrase 9 (CA9) in mouse samples, this Sandwich ELISA Kit uses a double-antibody, colorimetric format suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. CA9, also known as Carbonic Anhydrase IX and by the synonyms CAIX, CA-IX, and MN, is a key enzyme involved in regulating pH levels and facilitating tumor cell survival under hypoxic conditions, making it a valuable biomarker for cancer research and drug development.

The assay delivers a sensitivity of 9.375 pg/mL with a working range of 15.625-1000 pg/mL and is referenced to the mouse protein (UniProt Q8VHB5). Linearity has been validated across serum (92-102%, 85-101%, 86-97% at 1:2, 1:4, 1:8 dilutions), EDTA plasma (90-105%, 83-100%, 82-95%), and heparin plasma (90-105%, 87-101%, 83-94%). Recovery is consistently high, with averages of 97% for serum, 98% for EDTA plasma, and 92% for heparin plasma across their respective ranges (89-103%, 86-105%, 87-100%). The kit is intended for storage at 2-8°C for 12 months and offers a reliable, easy-to-use workflow for researchers studying the role of CA9 in cancer biology and potential therapeutic interventions.