Mouse CA125 ELISA Kit

Manufactured by Assay Genie, this sandwich (double antibody) ELISA kit enables quantitative measurement of CA125 in mouse serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. CA125 is a key biomarker associated with ovarian cancer and other gynecological diseases, and the kit supports research into disease monitoring and treatment evaluation.

The assay achieves a sensitivity of 1.875 IU/mL with a measuring range of 3.125–200 IU/mL. Detection relies on a biotinylated detection antibody and HRP-conjugated streptavidin, with TMB substrate producing a colorimetric change read at 450 nm. Validated linearity spans 80–105% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with recovery ranges of 85–105% and averages of 95% (serum), 94% (EDTA plasma), and 96% (heparin plasma).

The kit includes a pre-coated dismountable microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, Bradford reagent, and a technical manual. Reagents should be stored at 2–8°C for 12 months.