Mouse C5a/Complement C5a ELISA Kit

C5a is a 74-amino-acid anaphylatoxin generated by complement activation, exerting potent pro-inflammatory and chemotactic activity through the C5a receptor on myeloid cells. This quantitative immunoassay from Assay Genie enables researchers to measure mouse C5a concentrations in serum and plasma samples, supporting studies in innate immunity, inflammatory disease models, sepsis research, and complement-mediated pathology.

The kit provides a microplate pre-coated with capture antibody specific for the C5a epitope, along with biotinylated detection antibody, streptavidin-HRP conjugate, and lyophilized recombinant mouse C5a standard for curve generation. Researchers typically load serum or plasma volumes of 50 µL per well in duplicate, with a working detection range of 15.6 to 1000 pg/mL and a minimum detectable dose of approximately 7.8 pg/mL under optimized conditions.

Unopened reagents should be refrigerated at standard cold-chain temperatures, with the reconstituted standard aliquoted and frozen to preserve activity across minimal freeze-thaw cycles. The 96-well plate configuration supports multiple standard curve points and unknown samples simultaneously, with chromogenic development proportional to bound analyte and read with a standard microplate spectrophotometer.

Each lot is validated for specificity against mouse complement components and exhibits negligible cross-reactivity with related anaphylatoxins such as C3a and C4a, helping researchers distinguish complement activation signatures in experimental samples. Intended for research use only, this ELISA format streamlines workflows in immunology, pharmacology, and biomedical investigation laboratories focused on the complement cascade.