Mouse C-X-C chemokine receptor type 5 (Cxcr5) ELISA Kit

Assay Genie offers a sandwich ELISA kit for the quantitative measurement of mouse C-X-C chemokine receptor type 5 (CXCR5) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay uses a colorimetric detection method and is reactive with mouse samples, with specificity for natural and recombinant mouse CXCR5.

Performance is characterised by a detection range of 31.2-2000pg/ml and a sensitivity of 15.72pg/mL, with an intra-assay CV of 8.3% and inter-assay CV of 10.1%. Dilutional linearity for serum falls within 89-114%, for EDTA plasma within 100-119%, and for heparin plasma within 91-118% across 1:2 to 1:16 dilutions. Recovery in serum averages 100% (range 94-106%) and in plasma averages 102% (range 96-108%).

CXCR5 is a cytokine receptor that binds to B-lymphocyte chemoattractant (BLC) and is involved in B-cell migration into B-cell follicles of spleen and Peyer's patches, with dysregulation implicated in autoimmune diseases, inflammatory disorders and cancer progression. The kit is intended for research use only and supports work in the immunology field.