Mouse C-type natriuretic/NPPC ELISA Kit

Designed for the quantitative measurement of C-type natriuretic peptide (NPPC) in mouse samples, this Competitive ELISA kit from Assay Genie employs a coated antigen format with colorimetric detection. Validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, the assay delivers a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL.

The assay protocol involves equilibrating TMB substrate for 30 minutes at room temperature, adding 50 µL of standard or sample and 50 µL of biotin-labeled antibody to each well, incubating at 37°C for 45 minutes, washing the plate five times with soak time of 1 minute per wash, developing color with TMB substrate in the dark at 37°C for 10-20 minutes, stopping the reaction (blue to yellow), and reading absorbance at 450 nm. Samples are prepared by centrifugation at 1000 × g for 15-20 minutes, with cell lysates spun at 10,000 rpm for 10 minutes and tissue homogenates at 5000 × g for 5 minutes.

NPPC, also known as CNP or C-type natriuretic peptide, is a key regulator of cardiovascular and renal function, playing a crucial role in blood pressure and fluid balance regulation. Elevated NPPC levels have been associated with various cardiovascular diseases, making this a valuable biomarker for related research. Reagent storage is 2-8°C (sealed), with cryopreservation to be avoided.