Mouse C-C motif chemokine 4 (Ccl4) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is intended for research use only and is designed for the quantitative measurement of mouse C-C motif chemokine 4 (CCL4), also known as Macrophage Inflammatory Protein-1 beta (MIP-1 beta), using a colorimetric detection method. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity for natural and recombinant mouse CCL4.

Reported performance characteristics include a detection range of 15.6-1000pg/mL, a sensitivity of 7.89pg/mL, an intra-assay CV of 6.1% and an inter-assay CV of 10.5%. Linearity was assessed across 1:2, 1:4, 1:8 and 1:16 dilutions in serum (90-100%, 86-98%, 115-125%, 102-112%), EDTA plasma (108-117%, 91-103%, 84-96%, 105-117%) and heparin plasma (80-89%, 101-110%, 83-91%, 99-108%). Recovery averages 98% for serum (range 92-104%) and 100% for plasma (range 94-106%).

CCL4 is described as a monokine with inflammatory and chemokinetic properties that binds to CCR5 and acts as a homodimer, belonging to the intercrine beta (chemokine CC) family. It is associated with the UniProt entry P14097, NCBI Gene ID 20303, and has a molecular weight of 10,168 Da. The kit supports research applications in immunology and the study of inflammatory responses, immune regulation, autoimmune disorders and cancer progression.