Mouse C-C motif chemokine 2 (Ccl2) ELISA Kit

Designed for the accurate quantification of C-C motif chemokine 2 (CCL2) levels in mouse serum, plasma, and cell culture supernatants, this Sandwich ELISA Kit from Assay Genie offers high sensitivity and specificity for immunology research. The assay detects natural and recombinant mouse CCL2, also known as monocyte chemoattractant protein 1 (MCP-1), a key chemokine involved in immune cell recruitment and inflammatory responses, with implications in processes such as atherosclerosis and autoimmune diseases.

Quantitative performance includes a detection range of 31.2-2000pg/mL and a sensitivity of 15.74pg/mL. Precision is reported as an intra-assay CV of 4.5% and an inter-assay CV of 7.7%. Linearity in serum at dilutions of 1:2, 1:4, 1:8, and 1:16 (N=5) spans 94-114%, with EDTA plasma ranging 90-110% and heparin plasma 81-114%. Recovery averages 99% for serum (range 93-105%) and 101% for plasma (range 95-107%).

The kit is suitable for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. CCL2 functions as a chemotactic factor that attracts monocytes but not neutrophils. For research use only.