Mouse Bone sialoprotein 2 (Ibsp) ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie designed for the precise and accurate detection of Bone sialoprotein 2 (IBSP) levels in mouse serum, plasma, and cell culture supernatants. The target protein, also referred to as Bone sialoprotein II, Cell-binding sialoprotein, Integrin-binding sialoprotein, and BSP II, is a key protein in bone mineralization and remodeling that regulates osteoblast differentiation and function, making it a valuable biomarker for studying bone-related diseases and disorders.

Validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, the assay delivers a detection range of 0.312-20ng/mL and a sensitivity of 0.163ng/mL, with intra-assay CV of 6.3% and inter-assay CV of 7.7%. Specificity is confirmed for natural and recombinant mouse Bone sialoprotein 2. Recovery testing yields an average of 89% (range 83-95%) in serum and 91% (range 85-97%) in plasma, with linearity demonstrated across serial dilutions in serum, EDTA plasma, and heparin plasma. The kit is for research use only.

Well-suited to research in bone biology, osteoporosis, osteogenesis, and other skeletal-related conditions, as well as studies within the metabolism research area, this assay targets IBSP, a secreted protein encoded by the gene Ibsp (UniProt Q61711). Functionally, IBSP binds tightly to hydroxyapatite, forms an integral part of the mineralized matrix, and promotes Arg-Gly-Asp-dependent cell attachment.