Mouse Bile salt sulfotransferase 1 (Sult2a1) ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of Mouse Bile salt sulfotransferase 1 (SULT2A1) in mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The target enzyme, also known by the synonyms Hydroxysteroid sulfotransferase, Sulfotransferase 2A1, ST, ST2A1 and Sta1, is localized to the cytoplasm and is catalogued under UniProt accession P52843 (NCBI Gene ID 20859) with a molecular weight of 33,213 Da.

The assay delivers a detection range of 0.156–10 ng/mL and a sensitivity of 0.052 ng/mL. Reported linearity values for serum (N=5) span 82–117% across 1:2 to 1:16 dilutions, EDTA plasma (N=5) spans 92–118%, and heparin plasma (N=5) spans 93–120%. The kit is specific for natural and recombinant mouse Bile salt sulfotransferase 1 and is intended for research use only within the cardiovascular research area.

SULT2A1 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as a sulfonate donor to catalyze sulfonation of hydroxysteroids and xenobiotics, playing a role in bile acid metabolism and detoxification in the liver. Dysregulation of the enzyme has been linked to liver diseases and metabolic disorders, making it a relevant biomarker for related research.