Mouse BH3 Interacting Domain Death Agonist (Bid) ELISA Kit

Mitochondrial apoptosis research in mouse models frequently centers on Bid, a BH3-only Bcl-2 family member that bridges extrinsic death receptor signaling to the intrinsic mitochondrial pathway. Upon activation of cell surface Fas or TNF-family receptors, caspase-8 cleaves full-length Bid into its truncated form (tBid), which then translocates to mitochondrial membranes and engages Bax and Bak to promote cytochrome c release. Quantifying Bid concentrations across diverse specimen types therefore gives researchers a direct readout of apoptotic pathway activation in drug-induced cytotoxicity screens, liver injury studies, tumor chemotherapy response models, and neurodegeneration investigations.

This 96-assay ELISA kit from Assay Genie reacts with mouse Bid and accommodates varied upstream sample preparations, including tissue homogenates, cell lysates, and cell culture supernatants harvested from in vitro experiments. Whole-blood fractions such as serum-derived and plasma-derived specimens are likewise compatible, giving laboratories flexibility across both animal-model tissue analysis and cell-based assay pipelines. The microplate-based protocol is intended for research use only and is commonly deployed to track Bcl-2 family dynamics, caspase-8 cleavage events, mitochondrial outer membrane permeabilization, and downstream effector caspase activity in apoptosis-focused research programs.