Mouse Betaine--homocysteine S-methyltransferase 1 (Bhmt) ELISA Kit

Assay Genie's sandwich ELISA kit is designed for the measurement of Betaine-homocysteine S-methyltransferase 1 (BHMT) in mouse samples, with high sensitivity and specificity for precise and reproducible results. The assay supports research into the methionine cycle, methyl group metabolism, and homocysteine regulation, and is suitable for studies of metabolic disorders and the impact of dietary interventions on methyl group metabolism in mouse models.

Compatible sample types include serum and plasma, with the source protocol additionally describing preparation of cell culture supernatants, cell lysates, urine, cerebrospinal fluid, and tissue homogenates. BHMT is an important enzyme involved in the methionine cycle, playing a critical role in regulating homocysteine levels and methyl group metabolism. Dysregulation of BHMT has been linked to various health conditions, including cardiovascular diseases, liver dysfunction, and neurological disorders, making it a valuable marker for research purposes.

Detection is performed using a colorimetric sandwich ELISA format, with absorbance read at 450 nm. The kit is supplied with a removable microplate, lyophilized standard, sample and assay diluents, detection reagents, wash buffer, substrate, and stop solution. Reagents are stored at -20°C or 4°C as specified by component, and the kit is for research use only.