Mouse Beta-secretase 1 (Bace1) ELISA Kit

A colorimetric sandwich ELISA from Assay Genie designed for the quantitative detection of mouse Beta-secretase 1 (BACE1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. BACE1 is an aspartic protease that cleaves amyloid precursor protein (APP) at the N-terminus of the Aβ peptide sequence, between residues 671 and 672 of APP, a key step in the generation and extracellular release of beta-cleaved soluble APP and a corresponding cell-associated C-terminal fragment, with implications for the production of beta-amyloid peptides implicated in the pathogenesis of Alzheimer's disease and other neurodegenerative disorders.

The assay is specific for natural and recombinant mouse Beta-secretase 1 (UniProt P56818) and is intended for research use only within the neurosciences research area. Reported performance specifications include a detection range of 0.78–50 ng/mL, a sensitivity of 0.39 ng/mL, an intra-assay CV of 6.2% and an inter-assay CV of 8.1%; serum recovery averages 100% (range 94–106%) and plasma recovery averages 102% (range 96–108%).