Mouse Beta-nerve growth factor (Ngf) ELISA Kit

Assay Genie supplies a colorimetric Sandwich ELISA Kit for the quantitative measurement of mouse Beta-nerve growth factor (NGF), a protein recognised for its role in the development and maintenance of the sympathetic and sensory nervous systems. The assay is intended for research use only and is designed to work with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for both natural and recombinant mouse Beta-nerve growth factor.

The manufacturer reports a detection range of 15.6-1000 pg/mL and a sensitivity of 7.8 pg/mL, with an intra-assay CV of 4.8% and an inter-assay CV of 8.1%. Linearity has been evaluated in serum, EDTA plasma, and heparin plasma at dilutions from 1:2 to 1:16, and recovery averages 86% (range 80-92%) for serum and 88% (range 82-94%) for plasma.

Beta-nerve growth factor is described as an extracellular ligand for the NTRK1 and NGFR receptors that activates cellular signalling cascades regulating neuronal proliferation, differentiation, and survival. The protein is a secreted homodimer with a molecular weight of 27,077 Da, referenced under UniProt accession P01139.