Mouse BAFFR (B-Cell Activation Factor Receptor) CLIA Kit (MOES00111)

Assay Genie's Mouse BAFFR CLIA Kit leverages an antibody-pair sandwich configuration in which a capture antibody specific for the murine TNFRSF13C extracellular domain is immobilized on the well surface, and a detection antibody conjugated to a chemiluminescent acridinium ester label produces signal proportional to BAFFR concentration. The luminescent readout is captured on a compatible plate-reader luminometer, offering a broader dynamic range and lower background than colorimetric ELISA in many matrices. Researchers studying the BAFF/APRIL axis can pair this kit with parallel measurements of serum BAFF or APRIL to explore ligand-receptor stoichiometry in lupus-prone strains such as MRL/lpr or NZB/W F1 mice. The 96-well microplate is supplied pre-blocked and pre-coated, and the standard curve is generated from a lyophilized recombinant mouse BAFFR antigen that is reconstituted and serially diluted; intra-assay and inter-assay precision are typically reported with coefficients of variation below 10% for experienced users. Recommended sample volumes are 50 µL per well for serum and EDTA plasma, with hemolyzed or lipemic specimens flagged as potentially interfering. The assay performs well alongside flow cytometry phenotyping of splenic B-cell subsets, providing a quantitative serum biomarker readout that complements cellular immunophenotyping. Component shelf life is approximately 6 months from receipt when stored refrigerated at 2-8 °C; standards and detection conjugate should be kept in single-use aliquots to limit freeze-thaw cycles, and the kit is intended for research use only in vitro applications.