Mouse B-cell lymphoma/leukemia 11A (Bcl11a) ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA is intended for research use only and is designed for the accurate and sensitive detection of mouse B-cell lymphoma/leukemia 11A (Bcl11a) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay is reactive with mouse samples and is positioned as an essential tool for researchers studying B-cell lymphoma, leukemia and related disorders in mouse models.

Quantitative performance specifications include a detection range of 0.312-20ng/mL, a sensitivity of 0.172ng/mL, an intra-assay CV of 6.3% and an inter-assay CV of 8.2%. Linearity has been characterised in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16. Recovery has been measured at 95% (range 89-101%) for serum and 97% (range 91-103%) for plasma. The kit specifically recognises natural and recombinant mouse Bcl11a, a 26kDa protein referenced under UniProt entry Q9QYE3.

Bcl11a is reported to function as a myeloid and B-cell proto-oncogene with roles in leukemogenesis, hematopoiesis and lymphopoiesis, and is described as required for B-cell formation in fetal liver. The protein is noted as a modulator of the transcriptional repression activity of ARP1 and interacts with TFCOUP1, PIAS3, ARP1 and EAR2. It localises to the cytoplasm and nucleus, associates with the nuclear body, and colocalises with SUMO1 and SENP2 in nuclear speckles.