Mouse ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

The ATP1b1 protein serves as the beta-1 subunit of the heterodimeric Na+/K+-ATPase, anchoring the catalytic alpha subunit to the plasma membrane and stabilizing its conformation for efficient sodium and potassium ion pumping. This Assay Genie ELISA kit quantitatively measures mouse ATP1b1 expression across tissues such as kidney, heart, brain, and cochlea, supporting research into renal sodium handling, cardiac contractility, neuronal excitability, and auditory hair cell physiology, where ATP1b1 loss-of-function mutations are linked to sensorineural deafness. The kit includes a pre-coated microplate strip plate, lyophilized standards, detection antibody, wash buffer, and TMB substrate, with a required sample volume of 100 uL per determination run in duplicate. Reagents should be stored refrigerated and protected from light, with the standard and conjugate aliquoted upon arrival to limit freeze-thaw cycles; allow all components to equilibrate to room temperature before initiating the protocol. Intended for research use only, this mouse-specific immunoassay enables reproducible quantification of ATP1b1 in mouse models of hypertension, ischemia-reperfusion injury, and epithelial ion transport disorders.