Mouse ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit

Quantitative measurement of the gastric proton pump beta subunit in mouse samples is supported by this two-antibody ELISA from Assay Genie. The kit targets ATP4b, a glycosylated membrane protein of approximately 33 kDa that pairs with the alpha subunit to form the H+/K+ ATPase responsible for acid secretion by parietal cells in the stomach lining. The microplate-based protocol accommodates tissue homogenates prepared from gastric mucosa, with absorbance-based readout. Each kit provides components sufficient for 96 determinations in a single-use microplate format, including pre-coated wells, detection antibodies, lyophilized standards, and assay buffers. Most kit components should be refrigerated upon receipt, with the conjugate and substrate aliquoted and stored frozen for long-term stability. Researchers apply this ELISA to studies of acid secretion regulation, proton pump inhibitor pharmacology, autoimmune gastritis models, and Helicobacter pylori-related gastric disease, where ATP4b levels serve as a marker of parietal cell mass and function.