Mouse ATP synthase subunit beta, mitochondrial (Atp5b) ELISA Kit

Designed for the precise measurement of ATP5B levels in mouse samples, this colorimetric Sandwich ELISA Kit from Assay Genie provides a reliable tool for research applications. The assay is reactive with mouse targets and is suitable for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Key performance characteristics include a detection range of 31.2-2000pg/mL and a sensitivity of 15.6pg/mL. Intra-assay CV is 6.8% and inter-assay CV is 9.4%. Linearity has been validated in serum, EDTA plasma, and heparin plasma across multiple dilutions. Recovery averages 84% for serum (range 80-90%) and 86% for plasma (range 80-92%). The kit is specific for natural and recombinant mouse ATP synthase subunit beta, mitochondrial (UniProt P56480).

ATP5B is a key component of the mitochondrial ATP synthase (F(1)F(0) ATP synthase or Complex V), which produces ATP from ADP using a proton gradient generated by the respiratory chain. The protein localizes to the inner mitochondrial membrane. Dysregulation of ATP5B has been implicated in metabolic disorders, neurodegeneration, and cancer, making this kit useful for research in neurosciences. For research use only.