Mouse ATF6 (Activating Transcription Factor 6) ELISA Kit (MOES01641)

ATF6 is a key transcription factor that orchestrates the unfolded protein response (UPR) following endoplasmic reticulum stress. Quantifying its circulating levels helps researchers investigate metabolic disease, neurodegeneration, and hepatic models where ER homeostasis is disrupted.

Assay Genie supplies this capture-antibody-based format for the mouse ATF6 isoform. Each kit provides a pre-coated microplate, biotinylated detection antibody, recombinant standards, and streptavidin-HRP conjugate. After TMB substrate addition and stop solution, absorbance values are read on a standard plate reader.

Researchers typically load 50 µL of standard or sample per well, run the assay in duplicate, and follow the ELISA workflow. Reconstituted reagents remain stable when aliquoted and stored frozen, though repeated freeze-thaw cycles should be avoided to preserve antibody activity.