Mouse AT1R (angiotensin ? receptor type 1) ELISA Kit (MOFI01393)

The Assay Genie Mouse AT1R ELISA Kit enables quantitative measurement of angiotensin II type 1 receptor levels in mouse research samples. AT1R is a G protein-coupled receptor that mediates the pressor effects of angiotensin II, making it a focal target in studies of blood pressure regulation, cardiac remodeling, vascular smooth muscle proliferation, and renal sodium handling.

This immunoassay is configured for use with mouse-derived serum or plasma specimens, supporting investigations into hypertension, heart failure progression, diabetic nephropathy, and the pharmacological action of angiotensin receptor blockers. Researchers studying transgenic AT1A or AT1B knockout strains, salt-sensitive rodent models, or angiotensinogen overexpression systems commonly require sensitive receptor-level readouts to complement downstream signaling work and tissue expression analyses.

The kit provides reagents sufficient for 96 determinations in a microplate layout, with all required buffers, standards, and detection antibody pre-formulated for direct use. A capture antibody immobilizes mouse AT1R from loaded samples, a biotinylated detection reagent completes the binding pair, and a chromogenic substrate yields absorbance proportional to receptor concentration in each well, which is then read on a standard microplate reader.

For laboratory handling, store kit components refrigerated and protect from light; allow all reagents to equilibrate to room temperature before beginning the protocol. The complete assay is intended for research use only and is not validated for clinical diagnostic procedures, making it a useful addition to cardiovascular, renal, and metabolic disease research pipelines focused on the renin-angiotensin axis.