Mouse Aspartate aminotransferase, mitochondrial (Got2) ELISA Kit

Designed for the quantitative measurement of mouse aspartate aminotransferase, mitochondrial (Got2) in research samples, this sandwich-format ELISA kit from Assay Genie uses a colorimetric detection method and is suited for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reactivity is specific to mouse samples, and the assay detects natural and recombinant forms of mouse aspartate aminotransferase, mitochondrial.

Reported performance characteristics include a detection range of 0.78-50 U/L and a sensitivity of 0.39 U/L. Intra-assay precision is 5.3% (CV) and inter-assay precision is 9.1% (CV). In dilution linearity tested from 1:2 to 1:16, observed values span 81-119% across serum, EDTA plasma, and heparin plasma matrices. Spiked recovery is 97-109% for serum (average 103%) and 99-111% for plasma (average 105%). Got2 catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid, plays a key role in amino acid metabolism, mediates metabolite exchange between mitochondria and cytosol, and facilitates the cellular uptake of long-chain free fatty acids. The protein is catalogued under UniProt P05202 and localizes to the mitochondrion matrix and cell membrane.

The kit is intended for research use only and is associated with cell biology applications.