Mouse ASGR1/Asialoglycoprotein Receptor 1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables the quantitative measurement of mouse Asialoglycoprotein Receptor 1 (ASGR1) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, using a colorimetric, double-antibody detection format. ASGR1 is described as a key receptor involved in the clearance of circulating glycoproteins, with dysregulation linked to liver diseases such as cirrhosis and hepatitis, and to other disorders including diabetes and cancer, making the kit a relevant tool for research into metabolic and immune functions.

The assay has a stated sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. Reported linearity for serum, EDTA plasma, and heparin plasma across 1:2, 1:4, and 1:8 dilutions falls between 82% and 100%, while recovery averages 93% in serum, 98% in EDTA plasma, and 94% in heparin plasma. The protocol uses a 90-minute primary incubation at 37°C, biotinylated detection antibody binding for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, and TMB color development, with absorbance read at 450 nm. Standard components are stored at 2–8°C for 12 months, with selected items stored at -20°C for longer-term stability.