Mouse ARNT/HIF-1 beta ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double-antibody, colorimetric format for the quantitative detection of mouse ARNT/HIF-1 beta. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL, with storage at 2-8°C for 12 months.

ARNT/HIF-1 beta is described as a key transcription factor in hypoxia responses, regulating genes involved in cell proliferation, metabolism, and angiogenesis, with dysregulation linked to cancer, cardiovascular disorders, and inflammatory conditions. The kit is positioned within the Epigenetics and Nuclear Signaling research area.

Reported linearity (n = 5) at 1:2, 1:4 and 1:8 dilutions: serum 91-99% / 93-105% / 91-99%; EDTA plasma 85-101% / 85-101% / 82-97%; heparin plasma 80-98% / 95-100% / 86-98%. Recovery (n = 5) ranges: serum 90-105% (average 96%); EDTA plasma 85-101% (average 94%); heparin plasma 94-103% (average 99%).