Mouse Argininosuccinate Synthetase 1 (ASS1) ELISA Kit

Argininosuccinate synthetase 1 (ASS1) sits at a critical junction between the urea cycle and arginine biosynthesis, catalyzing the ATP-dependent ligation of citrulline and aspartate. Researchers working in hepatic metabolism, urea cycle disorders such as citrullinemia, and ASS1-dependent cancer biology rely on quantitative immunoassays to track this enzyme across disease models. This mouse-reactive ELISA kit from Assay Genie provides that measurement capability across tissue homogenate and blood-derived samples.

Loss of ASS1 expression has been documented in hepatocellular carcinoma, prostate cancer, and arginine-auxotrophic tumors, where it creates metabolic vulnerability to arginine-depleting therapies. The kit accordingly supports studies of tumor auxotrophy, in vivo treatment responses, and cell culture experiments probing arginine pathway pharmacology. Mouse cohort work exploring non-alcoholic fatty liver disease, alcoholic liver injury, and citrullinemia models can track enzyme abundance alongside PCR transcript analysis and western blot confirmation of protein expression.

With 96 assays in each kit, the format supports medium-throughput mouse studies that require parallel sampling of multiple treatment groups, time points, and matched controls. The workflow spans in vitro cell culture characterization, in vivo pharmacology, and tissue-level interrogation of arginine metabolism. Research teams integrating metabolomics or transcriptomics with proteomic readouts use this kit to bridge genomic and functional data on ASS1 regulation.