Mouse Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

Researchers studying eicosanoid signaling and inflammatory disease pathways rely on this ALOX5 ELISA Kit from Assay Genie to quantify arachidonate-5-lipoxygenase protein in mouse tissue homogenates and cell lysates. ALOX5 is a calcium-dependent, non-heme iron dioxygenase that catalyzes the first two committed steps in leukotriene biosynthesis, converting arachidonic acid into 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and then into leukotriene A4. Because leukotrienes drive bronchoconstriction, vascular permeability, and neutrophil recruitment, ALOX5 expression is a focal point in models of asthma, allergic airway inflammation, atherosclerosis, and macrophage or neutrophil biology.

The 96-assay microplate format supports absorbance and standard curve analysis, with sufficient reagents for up to 96 individual determinations per kit. Each kit contains pre-coated plate wells, assay buffers, and detection reagents required for the in vitro quantification workflow. By enabling reproducible measurement of ALOX5 in mouse-derived samples, this kit supports investigations into gene regulation, enzyme induction, and pharmacologic modulation of the leukotriene pathway in basic and translational research settings.