Mouse Apoptotic protease-activating factor 1 (Apaf1) ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie for the quantitative measurement of mouse Apoptotic protease-activating factor 1 (Apaf-1). The kit is reactive with mouse samples and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant mouse Apaf-1. Per the manufacturer, it uses a colorimetric detection method, offers a detection range of 0.312–20 ng/mL and a sensitivity of 0.16 ng/mL, with an intra-assay CV of 6.4% and an inter-assay CV of 9.0%.

Validated performance data include linearity in serum (87–99% to 107–117% across 1:2–1:16 dilutions), EDTA plasma (101–111% to 105–115%), and heparin plasma (97–107% to 117–127%), plus recovery averaging 102% for serum (range 96–108%) and 104% for plasma (range 98–110%). The target is a cytoplasmic, monomeric protein of 139,896 Da (UniProt O88879) that oligomerizes into a heptameric apoptosome upon binding cytochrome c and dATP, mediating cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to activation of caspase-3 and apoptosis. Because APAF1 dysregulation is linked to cancer and neurodegenerative disorders, the kit is positioned within the neurosciences research area. For research use only.