Mouse Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit

This quantitative immunoassay from Assay Genie measures mouse Apoptotic Peptidase Activating Factor 1 (APAF1), a central scaffold protein of the intrinsic apoptosis pathway that nucleates the apoptosome by binding cytochrome c and pro-caspase-9 to drive caspase-3 activation. Researchers use this microplate-based kit to quantify APAF1 protein levels across mouse tissue homogenates and cell lysate preparations, supporting studies of programmed cell death in oncology, neurodegeneration, cardiovascular disease, and developmental biology models. Each kit provides sufficient reagents for 96 individual determinations in a single plate run, with absorbance readings captured on standard plate readers to generate a standard curve from serially diluted reference antigen. Sample handling typically involves lysing fresh or snap-frozen tissues in cold buffer with protease inhibitors, clearing by centrifugation, and diluting clarified supernatants in the provided assay diluent before loading duplicate aliquots onto pre-coated wells. Investigators studying mitochondrial pathway involvement in chemotherapy response, ischemic injury, or genetic knockout phenotypes rely on this assay to compare APAF1 expression across treatment groups, time points, or transgenic backgrounds. The kit is supplied in a single-use box format containing all buffers, detection reagents, and plate components needed to complete one full plate of mouse APAF1 quantification, intended for research use only.