Mouse Apoptosis regulator Bcl-2 (Bcl2) ELISA Kit

From Assay Genie, this colorimetric Sandwich ELISA kit is designed for the quantitative measurement of mouse Apoptosis regulator Bcl-2 in research samples. Bcl-2 is a key anti-apoptotic member of the Bcl-2 family that suppresses apoptosis by controlling mitochondrial membrane permeability and inhibiting caspase activity, and its dysregulation is implicated in cancer and autoimmune disorders. The kit supports apoptosis research in mouse models, with the listed research area of Neurosciences.

Performance characteristics include a detection range of 0.156–10 ng/mL and a sensitivity of 0.087 ng/mL. Precision is reported as an intra-assay CV of 6.3% and an inter-assay CV of 8.2%. Recovery is validated at an average of 99% (range 93–105%) in serum and 101% (range 95–107%) in plasma, with linearity demonstrated across 1:2 to 1:16 dilutions of serum, EDTA plasma, and heparin plasma samples.

The kit accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and shows specificity for natural and recombinant mouse Apoptosis regulator Bcl-2. The target protein (UniProt P10417) is associated with the mitochondrion outer membrane, the nuclear membrane, and the endoplasmic reticulum membrane. The product is for research use only.