Mouse Apoptosis-inducing factor 1, mitochondrial (Aifm1) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of mouse apoptosis-inducing factor 1, mitochondrial (AIFM1) in mouse samples. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids (S1, S2). AIFM1 is described as a key regulator of apoptosis that functions in mitochondrial function and cell death, and the kit is listed for research use only within the cancer research area (S1, S2).

Performance figures quoted from the manufacturer include a detection range of 78–5000 pg/ml and a sensitivity of 40.4 pg/mL (S1, S2). Reported intra-assay CV is 6.6% and inter-assay CV is 8.5% (S1, S2). Linearity across dilutions of 1:2 to 1:16 is given for serum (104–114% down to 83–93%), EDTA plasma (107–116% down to 107–116%) and heparin plasma (110–120% down to 102–115%) (S1, S2). Recovery averages 105% (range 99–111%) for serum and 107% (range 101–113%) for plasma (S1, S2). The detection method is described as colorimetric (C0), consistent with an ELISA-format sandwich assay (S1, S2).

Listed synonyms for the target include Programmed cell death protein 8, Aif and Pdcd8, with stated specificity for natural and recombinant mouse AIFM1 (S1, S2).