Mouse Apolipoprotein A4 (APOA4) ELISA Kit

Apolipoprotein A4 is a circulating lipid-carrier protein synthesized primarily in the small intestine, where it participates in intestinal lipid absorption, reverse cholesterol transport, and the regulation of food intake and glucose homeostasis. The Mouse Apolipoprotein A4 ELISA Kit from Assay Genie provides researchers with a quantitative tool for measuring endogenous APOA4 levels across multiple mouse specimen types, supporting studies of metabolic disease, atherosclerosis, and energy-balance research.

Quantitation is achieved through a colorimetric readout, with absorbance values interpolated against a standard curve generated with calibrated reference material supplied in the kit. The 96-assay microplate format provides sufficient throughput for cohort studies or multi-condition experimental designs, while the standard kit components — pre-coated microplate, assay diluent, detection reagent, wash buffer, substrate solution, and stop solution — allow processing of an entire plate in one experimental run.

Validated specimen sources include tissue homogenates, cell lysates, and cell culture supernatants, alongside standard blood-derived fractions such as plasma or serum, enabling use in whole-animal physiology experiments as well as in vitro cell-based work. Samples and reconstituted reagents should be handled cold and stored refrigerated when not in active use, with freeze-thaw cycles limited to maintain epitope integrity. The assay is intended for research use only and is not validated for diagnostic applications.