Mouse APO-H (Apolipoprotein H) ELISA Kit (MOES00726)

Beyond simple quantification, this sandwich ELISA supports investigation of beta-2-glycoprotein I's role as a cofactor in antiphospholipid antibody recognition, with documented use in lupus-prone mouse models such as NZW x BXSB F1 and MRL/lpr strains. Each kit ships with a pre-coated 96-well microplate strip plate, lyophilized recombinant mouse APO-H standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate, and stop solution. Standard curves are typically generated from a seven-point dilution series, with intra-assay precision generally under 10% CV and inter-assay precision under 12% CV when manufacturer protocols are followed. Recommended sample volume is 50–100 µL per well, and the usable dynamic range generally covers 0.5–40 ng/mL, allowing dilution of high-titer serum or plasma specimens into the readable window. Unopened kit components are stored at 2–8 °C; reconstituted standards should be aliquoted and kept frozen to avoid repeated freeze-thaw cycles, while unused strips return to the foil pouch with desiccant. The Assay Genie protocol is compatible with any standard microplate reader using a 450 nm primary filter and optional 570 nm or 630 nm wavelength correction, making integration into existing immunology, lipid metabolism, and thrombosis research workflows straightforward.