Mouse APEX Nuclease 1 (APEX1) ELISA Kit

Quantitative measurement of mouse APEX1 (Apurinic/Apyrimidinic Endodeoxyribonuclease 1) is supported by this 96-assay immunoassay kit from Assay Genie. APEX1, also referred to as APE1 or REF-1, executes the rate-limiting endonuclease step of the base excision repair (BER) pathway and additionally acts as a redox regulator of transcription factors such as NF-κB, p53, AP-1, and HIF-1α, linking DNA damage repair to oxidative stress signaling. The kit accepts tissue homogenates and cell lysates prepared from mouse sources, and the chromogenic reaction is read on a standard microplate reader against a supplied standard curve. Typical research applications include profiling APEX1 expression in tumor xenograft models, in brain tissue from neurodegeneration studies, and in cultured cell lines subjected to genotoxic challenge or oxidative insult. Reagents are supplied in a single-use kit format sufficient for 96 individual reactions, and the product insert shipped with the package should be consulted for component-specific storage temperature, shelf life, and freeze-thaw handling. For research use only.