Mouse APAF1 ELISA Kit

Assay Genie's sandwich ELISA kit provides a colorimetric, double-antibody method for quantifying Apoptotic Protease Activating Factor 1 (APAF1) in mouse-derived samples. The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is suited to research into programmed cell death and related disease mechanisms including cancer, neurodegenerative disorders, and autoimmune conditions.

Performance specifications include a sensitivity of 4.688 pg/mL and a working range of 7.813–500 pg/mL. Linearity has been demonstrated across 1:2 to 1:8 dilutions, with serum ranging from 81–103%, EDTA plasma from 82–98%, and heparin plasma from 81–101%. Recovery is 88–99% (94% average) in serum, 86–104% (94% average) in EDTA plasma, and 86–103% (93% average) in heparin plasma. Reagents are stored at 2–8°C and remain stable for 12 months.

The workflow uses a pre-coated plate with sequential incubations at 37°C, biotin-labelled detection antibody, HRP–streptavidin amplification, and TMB colour development read at 450 nm.