Mouse Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

The Mouse Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit from Assay Genie is a quantitative immunoassay for measuring ACE2 protein levels in mouse sample matrices including tissue homogenates, cell lysates, and cell culture supernatant, as well as standard liquid specimen types, with a detection range of 0.156-10 ng/mL. ACE2 is a zinc-dependent metallopeptidase and a critical regulator of the renin-angiotensin system, where it converts angiotensin II into angiotensin 1-7, balancing vasoconstriction and vasodilation pathways relevant to cardiovascular and renal research.

The assay uses a sandwich format with a capture antibody pre-coated on a microplate and a detection antibody working with a substrate solution. Each kit contains plate strips, standards, detection reagents, and buffer concentrates, along with a complete protocol insert. The spectrophotometric readout is measured against a standard curve generated from the supplied reference samples, allowing quantitative interpolation of ACE2 concentrations from a 100 µL sample volume across the working range of the assay.

Unopened kits are shipped refrigerated and should be stored at 4 degrees C until the expiration date; opened reagents are stable for short-term use and should be aliquoted and frozen at -20 degrees C for long-term storage to avoid repeated freeze-thaw cycles. The kit is intended for research use only and is not approved for diagnostic or clinical applications.