Mouse Angiopoietin 2 (ANGPT2) ELISA Kit

Angiopoietin-2 (ANGPT2) is a secreted glycoprotein ligand of the endothelial Tie-2 receptor, where it functions as a context-dependent antagonist that destabilizes quiescent vasculature and promotes angiogenic remodeling in settings such as tumor vascularization, wound repair, sepsis, and inflammatory disease. This quantitative immunoassay from Assay Genie is configured for research use only and enables researchers working with murine models to measure endogenous ANGPT2 protein levels across serum-derived samples, plasma-derived samples, and tissue homogenate preparations. The kit is supplied in a 96-assay format with all reagents required to generate a standard curve and run unknown samples in duplicate, and it pairs the capture antibody, detection antibody, and streptavidin-HRP chemistry needed to produce a signal proportional to ANGPT2 concentration in each reaction. Reconstituted standard and prepared samples should be handled according to the insert, with unused strips returned to the foil pouch to protect from light; working reagents should be stored refrigerated between uses, and frozen for long-term retention to preserve activity. Researchers studying vascular biology, oncology, ophthalmology, and metabolic disease in mice will find this assay useful for tracking dynamic changes in ANGPT2 expression in response to experimental interventions, comparing expression across genotypes or treatment groups, and validating findings from transcript-level or imaging-based readouts at the protein level.