Mouse Angiomotin/AMOT ELISA Kit

From Assay Genie, this sandwich ELISA is designed for the quantitative detection of Angiomotin (AMOT) in mouse samples, supporting research in cancer biology, cardiovascular disease, and angiogenesis. Angiomotin is described as a critical protein involved in regulating cell growth, migration, and cell junction stability, and is implicated in angiogenesis and tumour suppression.

The assay is configured as a double-antibody sandwich ELISA and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 46.875 pg/mL with a working range of 78.125-5000 pg/mL. Kits are stored at 2-8°C for 12 months.

Reported linearity across dilutions (1:2, 1:4, 1:8) for serum is 94-100%, 84-99%, 80-100%; EDTA plasma 90-105%, 84-97%, 89-99%; heparin plasma 86-101%, 84-95%, 84-95%. Recovery averages were 95% (serum, range 86-102%), 96% (EDTA plasma, range 85-104%), and 96% (heparin plasma, range 92-100%). The procedure is a six-step colourimetric workflow: reagent and plate preparation, 90-minute primary incubation at 37°C, biotin-labelled detection antibody for 60 minutes at 37°C, HRP-streptavidin (SABC) for 30 minutes at 37°C, TMB colour development in the dark for 10–20 minutes, then stop reaction with absorbance read at 450 nm.