Mouse Aminopeptidase N/CD13 ELISA Kit

Manufactured by Assay Genie, the Mouse Aminopeptidase N/CD13 ELISA Kit is a sandwich ELISA in a double-antibody format designed for the quantitative measurement of mouse Aminopeptidase N (CD13). The colorimetric assay employs a TMB substrate with absorbance read at 450 nm, and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The associated UniProt entry is P97449, and the kit is listed for cardiovascular research.

Aminopeptidase N (CD13) is a key enzyme involved in peptide and protein metabolism, with reported roles in inflammation, tumor progression and immune regulation. The kit reports a sensitivity of 0.469 ng/mL and an assay range of 0.781-50 ng/mL. Linearity at dilution ratios of 1:2, 1:4 and 1:8 is reported for serum (89-101%, 85-97% and 86-103%), EDTA plasma (83-99%, 88-100% and 82-94%) and heparin plasma (82-91%, 82-98% and 80-91%). Spike-and-recovery across n=5 averages 95% in serum (range 89-103%), 93% in EDTA plasma (range 86-98%) and 96% in heparin plasma (range 90-104%).

The kit includes a pre-coated microplate, lyophilized standard, biotin-labeled detection antibody, HRP-streptavidin conjugate, TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer and a plate sealer. Reagents are stored at 2-8°C for 12 months, with the standard also suitable for longer-term storage at -20°C.