Mouse alphaFP (Alpha-Fetoprotein) ELISA Kit (MOES01686)

Assay Genie’s Mouse alphaFP (Alpha-Fetoprotein) ELISA Kit is a colorimetric, sandwich-format ELISA for measuring mouse alpha-fetoprotein, also listed under the synonyms AFP, FETA, HPAFP and alpha-fetoglobulin. Samples specified include serum, plasma and other biological fluids, with cell culture supernatants also named as a sample type. Alpha-fetoprotein is described as a biomarker associated with liver development, liver disease and certain types of cancer, and the kit’s listed research areas are cancer, cardiovascular research and developmental biology.

The manufacturer reports a sensitivity of 0.1 ng/mL and a detection range of 0.16-10 ng/mL, with an assay time of 3 h 30 min. Recovery is listed as 80%-120%; in the recovery table, EDTA plasma shows 95-108% with an average recovery of 101%, while cell culture media shows 92-105% with an average recovery of 97%.

Sample volume is given as 100 μL, and linearity data cover serum, EDTA plasma and cell culture media at dilutions from 1:2 to 1:16. Intra-assay CVs are listed as 4.8%, 6.24% and 4.53% for the three samples tested, with inter-assay CVs of 7.85%, 7.45% and 7.0%. The kit recognises mouse αFP, with no significant cross-reactivity or interference observed between mouse αFP and analogues.