Mouse AlphaB Crystallin/CRYAB ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of mouse AlphaB-Crystallin (CryAB) in research samples. CryAB is a molecular chaperone protein that plays a critical role in protecting cells from stress and maintaining protein homeostasis, and its dysregulation has been implicated in neurodegenerative and cardiovascular diseases. Measuring CryAB levels can therefore provide valuable insights into disease mechanisms and potential therapeutic targets.

The kit accommodates a broad range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, based on a double-antibody sandwich format. The workflow involves equilibrating reagents and TMB substrate, loading standards and samples for primary incubation at 37°C, binding with a biotin-labeled detection antibody, adding HRP-streptavidin, developing with TMB substrate in the dark, and reading absorbance at 450 nm.

Storage is recommended at 2-8°C for 12 months, and the user-friendly protocol supports straightforward quantification of CryAB levels in mouse samples.