Mouse Alpha-Endorphin (aEP) ELISA Kit

The Mouse Alpha-Endorphin (aEP) ELISA Kit from Assay Genie is a competitive inhibition immunoassay built on a pre-coated microplate to quantify alpha-endorphin, a 16-residue endogenous opioid peptide cleaved from the proopiomelanocortin (POMC) precursor. Researchers in neurobiology, behavioral studies, addiction models, and pituitary or hypothalamic function investigations use this kit to characterize endogenous opioid tone in mouse samples.

Key assay specifications include a standard curve range of 31.25 to 2000 pg/mL, a sensitivity below 18.75 pg/mL, and a 50 µL sample volume per well using serum, plasma, or other biological fluids. The colorimetric readout is captured at 450 nm after a standard bench-top incubation, and the full assay run completes in roughly 2.5 hours.

For reliable performance, store kit components refrigerated at 2-8 °C and protect the antibody-coated strips from moisture; reconstituted standards should be aliquoted and frozen at -20 °C to limit freeze-thaw cycles. This product is intended for research use only and is not validated for diagnostic procedures.