Mouse alpha 5 Defensin/DEFA5 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise quantification of alpha 5 Defensin (DEFA5) levels in mouse samples, including serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. DEFA5 is described as a key component of the immune system, known for its antimicrobial properties and role in host defense, with alterations in its levels associated with inflammatory diseases, infectious conditions, and gut health.

The assay uses a double-antibody sandwich format with a reported sensitivity of 9.375 pg/mL and a detection range of 15.625-1000 pg/mL. The associated UniProt accession is P28312, with synonyms listed as Defa5 and Defensin-related cryptdin-5. Overall kit storage is 2-8°C for 12 months.

Reported linearity across dilution factors (1:2, 1:4, 1:8) includes: Serum (n=5) 85-105%, 90-99%, 99-104%; EDTA Plasma (n=5) 87-98%, 82-96%, 83-101%; Heparin Plasma (n=5) 84-97%, 81-98%, 81-100%. Reported recovery includes: Serum (n=5) 90-95% with average 91%; EDTA Plasma (n=5) 93-100% with average 97%; Heparin Plasma (n=5) 87-100% with average 93%.