Mouse alpha 1-Microglobulin ELISA Kit

Manufactured by Assay Genie, this Sandwich (double-antibody) ELISA is designed for the quantitative measurement of alpha-1-microglobulin in mouse samples using a colorimetric readout. The kit has a sensitivity of 0.938 ng/mL and a standard range of 1.563–100 ng/mL, with absorbance read at 450 nm after TMB color development. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the product is intended for research into conditions such as kidney disease, inflammatory disorders, and oxidative stress-related states where alpha-1-microglobulin serves as a biomarker of antioxidant activity and immune regulation.

Spike-and-recovery performance averages 96% in serum (range 86–105%), 94% in EDTA plasma (85–103%), and 96% in heparin plasma (89–104%). Linearity across 1:2, 1:4, and 1:8 dilutions remains within 81–104% for serum, 84–103% for EDTA plasma, and 84–103% for heparin plasma. The workflow uses a biotin-labelled detection antibody followed by HRP-streptavidin binding and TMB color development with incubation steps performed at 37°C, and all components are stored at 2–8°C (protected from light) for 12 months.