Mouse Alpha-1-acid glycoprotein 1 (Orm1) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of Mouse Alpha-1-acid glycoprotein 1 (ORM1), also referred to as Orosomucoid-1, OMD 1, AGP 1, Agp1 or Orm-1. The colorimetric assay has a detection range of 1.56-100ng/mL and a sensitivity of 0.89ng/mL, and is reactive with mouse samples.

Validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, the kit recognises both natural and recombinant mouse Alpha-1-acid glycoprotein 1. Reported intra-assay CV is 7.0% and inter-assay CV is 8.9%. Linearity in serum (N=5) ranges from 97-106% to 98-109% at 1:2 through 1:16 dilutions; EDTA plasma (N=5) shows 95-105% to 103-113% across the same dilutions, while heparin plasma (N=5) shows 83-83% to 102-112%. Average recovery is 97% (range 91-103%) for serum and 99% (range 93-105%) for plasma.

ORM1 is described as a secreted transport protein in the bloodstream that binds ligands within its beta-barrel domain and appears to function in modulating the activity of the immune system during the acute-phase reaction. The protein is classed as an acute-phase reactant relevant to immunology research, including studies of infection, inflammation and cancer. Standard, sample diluent, assay diluents and detection reagents are stored at -20°C, with wash buffer, substrate solution and stop solution stored at 4°C. For research use only.