Mouse Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit

The Mouse Alkaline Sphingomyelinase (Alk-SMase) ELISA Kit from Assay Genie targets the ENPP7-encoded enzyme that hydrolyzes sphingomyelin to produce ceramide at alkaline pH. Investigators apply this assay in studies of intestinal lipid metabolism, ceramide-mediated apoptosis, and lysosomal storage disorders including Niemann-Pick disease type C. The microplate format yields quantitative readouts from cell lysates and tissue homogenates, with each vial of standards, detection antibody, and substrate supplied ready to use. Reagents should be refrigerated upon arrival and protected from light; bring all components to room temperature before running the assay. Run time is approximately 3.5 hours, with sample volume around 50 µl per well. For research use only.