Mouse Alkaline Phosphatase/ALPL ELISA Kit

From Assay Genie, this Sandwich ELISA Kit uses a double-antibody format and a colorimetric detection method to quantify mouse alkaline phosphatase (ALPL) levels. It is designed for use with serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The assay has a reported sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. Alkaline phosphatase is an enzyme involved in biological processes such as bone mineralization and cell signaling, and abnormal levels have been linked to liver and bone disorders, making ALPL a useful target for research in these areas.

Linearity has been validated at dilutions of 1:2, 1:4 and 1:8 in serum (89–102%, 87–101%, 92–99%), EDTA plasma (89–98%, 83–101%, 82–99%) and heparin plasma (80–93%, 85–97%, 81–91%). Reported spike-and-recovery ranges are 88–105% (average 97%) for serum, 85–105% (average 100%) for EDTA plasma, and 86–98% (average 93%) for heparin plasma. The kit is intended to be stored at 2–8°C for 12 months.