Mouse AGRP/Agouti-related protein ELISA Kit

For researchers investigating obesity, metabolism, and related conditions in mouse models, Assay Genie offers a Mouse AGRP sandwich ELISA kit designed for the precise measurement of agouti-related protein levels. The assay is reactive with mouse serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. AGRP plays a key role in regulating feeding behavior and energy balance in mammals, making it a valuable biomarker for studies of obesity and metabolic disorders.

Quantitative performance specifications include a sensitivity of 9.375 pg/mL and a standard range of 15.625-1000 pg/mL. Linearity validation in serum (n = 5) was 94-102% at 1:2, 87-100% at 1:4, and 89-99% at 1:8; EDTA plasma (n = 5) was 86-105%, 89-101%, and 91-100%; heparin plasma (n = 5) was 89-105%, 89-99%, and 88-98%. Recovery ranges were 86-104% (serum, average 94%), 85-105% (EDTA plasma, average 96%), and 86-103% (heparin plasma, average 94%). The assay uses a biotin-labeled detection antibody with HRP-Streptavidin conjugate and TMB substrate, with absorbance read at 450 nm. Kit components should be stored at 2-8°C for 12 months.